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Anti-EEF2K Antibody
Catalog #: 107-10972
EEF2K antibody [N1N3], 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 29904 |
| Protein Name / Synonyms | EEF2K |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
HSU93850 antibody, MGC45041 antibody, eEF-2K antibody, EEF2K antibody, eEF-2 kinase antibody, eukaroytic elongation factor 2 kinase antibody, calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase antibody, calmodulin-dependent protein kinase III antibody, eukaryotic elongation factor 2 kinase antibody, calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase antibody, elongation factor-2 kinase antibody, eukaryotic elongation factor-2 kinase antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
82 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 268 of EEF2K (Uniprot ID# O00418)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using EEF2K(107-10972) antibody at 1:500 dilution.
Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
107-10972 diluted at 1:1000
A: mouse liver
7.5% SDS PAGE
107-10972 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed A431, using EEF2K(107-10972) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: A431
B: H1299
C: Hela
D: Hep G2
7.5% SDS PAGE
107-10972 diluted at 1:5000
A: A431
B: H1299
C: Hela
D: Hep G2
7.5% SDS PAGE
107-10972 diluted at 1:5000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.