EFTUD2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q15029 |
| Gene Id | 9343 |
| Gene Symbols | EFTUD2 |
| Protein Name / Synonyms | EFTUD2, MFDGA, MFDM, SNRNP116, Snrp116, Snu114, U5-116KD, 116 kDa U5 small nuclear ribonucleoprotein component |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a GTPase which is a component of the spliceosome complex which processes precursor mRNAs to produce mature mRNAs. Mutations in this gene are associated with mandibulofacial dysostosis with microcephaly. Multiple transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:20 - 1:50
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:20 - 1:50
Format
Calculated MW
105kDa/108kDa/109kDa
Observed MW
120kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human EFTUD2 (NP_004238.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EFTUD2 antibody (144-07040) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 20s.
Immunohistochemistry of paraffin-embedded mouse brain using EFTUD2 Antibody (144-07040) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using EFTUD2 Antibody (144-07040) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using EFTUD2 Antibody (144-07040) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EFTUD2 Antibody (144-07040) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EFTUD2 Antibody (144-07040) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using EFTUD2 Antibody (144-07040) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using EFTUD2 antibody (144-07040).
Immunoprecipitation analysis of 100ug extracts of 293T cells using 3ug EFTUD2 antibody (144-07040). Western blot was performed from the immunoprecipitate using EFTUD2 antibody (144-07040) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.