Anti-EGFR Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Accession Number	P00533
Gene Id	1956
Gene Symbols	EGFR
Protein Name / Synonyms	EGFR
Clonality	Monoclonal
Preparation	Purified His-tagged EGFR protein(Fragment) was used to produced this monoclonal antibody.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	FC, IHC-P, Western Blotting
Research Area	Cardiovascular Disease, Cancer, Inflammation, HER/ErbB Signaling, Tyrosine Kinase Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.

Specificity

Other Names

EGFR; ERBB; ERBB1; HER1; Epidermal growth factor receptor; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1

NCBI Accession #

NP_005219.2;NP_958439.1;NP_958440.1;NP_958441.1

Format

Type

Purified Mouse Monoclonal Antibody (Mab)

Calculated Molecular Weight (Da)

134277

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of lysate from A431 cell line, using EGFR Antibody(Cat# 102-10505). 102-10505 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug.
	Formalin-fixed and paraffin-embedded human breast carcinoma with EGFR Monoclonal Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of HepG2 cells using EGFR Monoclonal Antibody(bottom histogram) compared to a negative control cell (top histogram). PE-conjugated goat-anti-mouse secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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