EGFR Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P00533 |
| Gene Id | 1956 |
| Gene Symbols | EGFR |
| Protein Name / Synonyms | EGFR, ERBB, ERBB1, HER1, NISBD2, PIG61, mENA, epidermal growth factor receptor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Cardiovascular Disease, Cancer, Inflammation, HER/ErbB Signaling, Tyrosine Kinase Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
44kDa/69kDa/77kDa/134kDa
Observed MW
160kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1021-1210 of human EGFR (NP_005219.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EGFR antibody (144-11351) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.![Western blot - [KO Validated] EGFR Polyclonal Antibody (144-11351)](https://cdn.sanity.io/images/g51potcz/production/e2bce80115127798e3775381bc097978bacd1032-407x350.jpg)
Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells, using EGFR antibody (144-11351) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.![Western blot - [KO Validated] EGFR Polyclonal Antibody (144-11351)](https://cdn.sanity.io/images/g51potcz/production/dd65a0cfbcfbb25582f4042c69f99961549e904f-350x350.jpg)
Immunohistochemistry of paraffin-embedded rat lung using EGFR antibody (144-11351) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] EGFR Polyclonal Antibody (144-11351)](https://cdn.sanity.io/images/g51potcz/production/dc3e5ca5d0119279d11650524304897e6640e6f1-350x350.jpg)
Immunohistochemistry of paraffin-embedded human prostate using EGFR antibody (144-11351) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] EGFR Polyclonal Antibody (144-11351)](https://cdn.sanity.io/images/g51potcz/production/66ba1635dc57dc3c06a33152fb5b77b6abe5c7cb-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse brain using EGFR antibody (144-11351) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] EGFR Polyclonal Antibody (144-11351)](https://cdn.sanity.io/images/g51potcz/production/d3483121d1a8bf6a9d2819514d65cd43fa5b21af-350x350.jpg)
Immunohistochemistry of paraffin-embedded mouse heart using EGFR antibody (144-11351) at dilution of 1:100 (40x lens).![Immunohistochemistry - [KO Validated] EGFR Polyclonal Antibody (144-11351)](https://cdn.sanity.io/images/g51potcz/production/0961d6e4d586111f0bd0c804d0888ebcf734bbf9-440x550.jpg)
Immunofluorescence analysis of HeLa cells using EGFR antibody (144-11351) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (144-11351)](https://cdn.sanity.io/images/g51potcz/production/680af5e5fce69f2e4b880b5e5636a5649a21b415-440x550.jpg)
Immunofluorescence analysis of NIH/3T3 cells using EGFR antibody (144-11351) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (144-11351)](https://cdn.sanity.io/images/g51potcz/production/7eb7495e735e2a1dfe49b7076daeeebc8676e20a-440x550.jpg)
Immunofluorescence analysis of PC12 cells using EGFR antibody (144-11351) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunofluorescence - [KO Validated] EGFR Polyclonal Antibody (144-11351)](https://www.raybiotech.com/files/images/Antibodies/144/144-11351/9.jpg)
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug EGFR antibody (144-11351). Western blot was performed from the immunoprecipitate using EGFR antibody (144-11351) at a dilition of 1:1000.![Immunoprecipitation - [KO Validated] EGFR Polyclonal Antibody (144-11351)](https://www.raybiotech.com/files/images/Antibodies/144/144-11351/10.jpg)
Expiration:
12 months from the date of shipment when stored properly.
