Anti-EGFR (Y869) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P00533\|Q01279
Gene Id	1956
Gene Symbols	EGFR
Protein Name / Synonyms	Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Clonality	Polyclonal
Clone	RB10970
Isotype	Kappa, Rabbit
Recommended Applications	Western Blotting
Research Area	Cancer, HER/ErbB Signaling, Tyrosine Kinase Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. A prototype member of the type 1 receptor tyrosine kinases, EGFR is encoded by the cellular oncogene cerbB1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxy terminal domain. The carboxy terminal domain contains at least four tyrosine autophosphorylation sites. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

Other Information

NCBI Accession #

NP_005219.2;NP_958439.1;NP_958440.1;NP_958441.1

Antigen Type

Synthetic Peptide

Format

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Calculated Molecular Weight (Da)

134277

Antigen Source

Human

Images

WB (1:1000)

All lanes : Anti-EGFR Antibody (Y869) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: MDA-MB-231 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 134 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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