Anti-EGLN2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q96KS0
Gene Id	112398
Gene Symbols	EGLN2
Protein Name / Synonyms	EGLN2, EIT6, HIF-PH1, HIFPH1, HPH-1, HPH-3, PHD1, egl nine homolog 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene.

Dilutions

WB 1:500 - 1:2000
IP 1:20 - 1:50

Format

Calculated MW

40kDa/43kDa

Observed MW

43kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human EGLN2 (NP_444274.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using EGLN2 antibody (144-02252) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - EGLN2 Polyclonal Antibody (144-02252)

Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug EGLN2 antibody (144-02252). Western blot was performed from the immunoprecipitate using EGLN2 antibody (144-02252) at a dilition of 1:1000.
Immunoprecipitation - EGLN2 Polyclonal Antibody (144-02252)

Expiration:

12 months from the date of shipment when stored properly.

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