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Anti-EHHADH (C-term) Antibody

Catalog #: 102-17959

Rabbit Anti-EHHADH (C-term) Antibody, 400 ul

Product Description

Specifications

Size400 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
Q08426
Gene Id
1962
Gene Symbols
EHHADH
Protein Name / Synonyms
EHHADH (C-term)
ClonalityPolyclonal
PreparationThis EHHADH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 662-690 amino acids from the C-terminal region of human EHHADH.
Concentration (lot specific)
0.5 mg/ml
Recommended ApplicationsWestern Blotting, IHC-P, FC
Shipping TypeBlue ice
Storage-20°C

Description

Introduction

EHHADH is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity.

Specificity

Predicted Reactivity
Rat
Other Names
EHHADH; ECHD; Peroxisomal bifunctional enzyme; Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase; 3-hydroxyacyl-CoA dehydrogenase
NCBI Accession #
NP_001159887.1;NP_001957.2
Other Accession #
P07896; Q9DBM2

Format

Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
79495
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
Western blot analysis of EHHADH Antibody (C-term) (Cat# 102-17959) in mouse liver(lane 1), kidney(lane 2) tissue lysates (35ug/lane). EHHADH (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with EHHADH Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of HepG2 cells using EHHADH Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.