Anti-EHMT1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9H9B1
Gene Id	79813
Gene Symbols	EHMT1
Protein Name / Synonyms	EHMT1, EUHMTASE1, Eu-HMTase1, FP13812, GLP, GLP1, KMT1D, bA188C12.1, histone-lysine N-methyltransferase EHMT1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Cardiovascular Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a histone methyltransferase that is part of the E2F6 complex, which represses transcription. The encoded protein methylates the Lys-9 position of histone H3, which tags it for transcriptional repression. This protein may be involved in the silencing of MYC- and E2F-responsive genes and therefore could play a role in the G0/G1 cell cycle transition. Defects in this gene are a cause of chromosome 9q subtelomeric deletion syndrome (9q-syndrome, also known as Kleefstra syndrome). Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

7kDa/86kDa/128kDa/141kDa

Observed MW

141kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human EHMT1 (NP_079033.4).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using EHMT1 antibody (144-08513) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Western blot - EHMT1 Polyclonal Antibody (144-08513)

Expiration:

12 months from the date of shipment when stored properly.

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