EHMT2 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q96KQ7 |
| Gene Id | 10919 |
| Gene Symbols | EHMT2 |
| Protein Name / Synonyms | EHMT2, BAT8, C6orf30, G9A, GAT8, KMT1C, NG36, histone-lysine N-methyltransferase EHMT2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a methyltransferase that methylates lysine residues of histone H3. Methylation of H3 at lysine 9 by this protein results in recruitment of additional epigenetic regulators and repression of transcription. This gene was initially thought to be two different genes, NG36 and G9a, adjacent to each other in the HLA locus. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
19kDa/129kDa/132kDa
Observed MW
132kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 877-1176 of human EHMT2 (NP_079532.5).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EHMT2 antibody (144-01247) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Expiration:
12 months from the date of shipment when stored properly.
