Anti-Eif2ak2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P19525
Gene Id	5610
Gene Symbols	EIF2AK2
Protein Name / Synonyms	EIF2AK2, EIF2AK1, PKR, PPP1R83, PRKR, eukaryotic translation initiation factor 2 alpha kinase 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:100 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

57kDa/62kDa

Observed MW

73kDa, 80kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human Eif2ak2 (NP_002750.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using Eif2ak2 antibody (144-05578) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - [KO Validated] Eif2ak2 Polyclonal Antibody (144-05578)

Western blot analysis of extracts from normal (control) and EIF2AK2 knockout (KO) HeLa cells, using EIF2AK2 antibody (144-05578) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] Eif2ak2 Polyclonal Antibody (144-05578)

Immunohistochemistry of paraffin-embedded human liver using Eif2ak2 antibody (144-05578) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] Eif2ak2 Polyclonal Antibody (144-05578)

Immunofluorescence analysis of U2OS cells using EIF2AK2 antibody (144-05578) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] Eif2ak2 Polyclonal Antibody (144-05578)

Expiration:

12 months from the date of shipment when stored properly.

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