EIF2S3 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P41091 |
| Gene Id | 1968 |
| Gene Symbols | EIF2S3 |
| Protein Name / Synonyms | EIF2S3, EIF2, EIF2G, EIF2gamma, MRXSBRK, eIF-2gA, eukaryotic translation initiation factor 2 subunit 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is the largest subunit of a heterotrimeric GTP-binding protein involved in the recruitment of methionyl-tRNA(i) to the 40 S ribosomal subunit.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
51kDa
Observed MW
45kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 173-472 of human EIF2S3 (NP_001406.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EIF2S3 antibody (144-06581) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver using EIF2S3 antibody (144-06581) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using EIF2S3 antibody (144-06581) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using EIF2S3 antibody (144-06581) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.