Anti-EIF3H (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	O15372
Gene Id	8667
Gene Symbols	EIF3H
Protein Name / Synonyms	EIF3H (C-term)
Clonality	Polyclonal
Preparation	This EIF3H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 234-263 amino acids from the C-terminal region of human EIF3H.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

EIF3H is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Specificity

Predicted Reactivity

Bovine, Chicken, Xenopus

Other Names

EIF3H; EIF3S3; Eukaryotic translation initiation factor 3 subunit H; Eukaryotic translation initiation factor 3 subunit 3; eIF-3-gamma; eIF3 p40 subunit

NCBI Accession #

NP_003747.1

Other Accession #

Q5PPY6; Q5ZLE6; Q56JZ5

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

39930

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	EIF3H Antibody (N-term) (Cat# 102-16859) western blot analysis in Hela cell line and mouse bladder tissue lysates (35ug/lane).This demonstrates the EIF3H antibody detected the EIF3H protein (arrow).
	Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with EIF3H Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of hela cells using EIF3H Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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