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Anti-EIF4A1 Antibody
Catalog #: 144-05294
EIF4A1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P60842 |
| Gene Id | 1973 |
| Gene Symbols | EIF4A1 |
| Protein Name / Synonyms | EIF4A1, DDX2A, EIF-4A, EIF4A, eIF-4A-I, eIF4A-I, eukaryotic initiation factor 4A-I |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:2000
IF 1:20 - 1:50
IP 1:20 - 1:50
IF 1:20 - 1:50
IP 1:20 - 1:50
Format
Calculated MW
39kDa/46kDa
Observed MW
48kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-406 of human EIF4A1 (NP_001407.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EIF4A1 antibody (144-05294) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunofluorescence analysis of MCF-7 cells using EIF4A1 antibody (144-05294). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.