3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-EIF4B Antibody
Catalog #: 144-05405
EIF4B Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P23588 |
| Gene Id | 1975 |
| Gene Symbols | EIF4B |
| Protein Name / Synonyms | EIF4B, EIF-4B, PRO1843, eukaryotic translation initiation factor 4B |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:100
IP 1:50 - 1:200
Format
Calculated MW
64kDa/69kDa
Observed MW
85kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 352-611 of human EIF4B (NP_001408.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EIF4B antibody (144-05405) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human liver cancer using EIF4B Antibody (144-05405) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human prostate using EIF4B Antibody (144-05405) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using EIF4B Antibody (144-05405) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using EIF4B antibody (144-05405). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug EIF4B antibody (144-05405). Western blot was performed from the immunoprecipitate using EIF4B antibody (144-05405) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.