Anti-EIF4EBP1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q13541
Gene Id	1978
Gene Symbols	EIF4EBP1
Protein Name / Synonyms	EIF4EBP1, 4E-BP1, 4EBP1, BP-1, PHAS-I, eukaryotic translation initiation factor 4E-binding protein 1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	mTOR Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Dilutions

WB 1:500 - 1:1000
IF 1:50 - 1:200

Format

Calculated MW

12kDa

Observed MW

20kDa, 22kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human EIF4EBP1 (NP_004086.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using EIF4EBP1 antibody (144-01248) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Western blot - [KO Validated] EIF4EBP1 Polyclonal Antibody (144-01248)

Western blot analysis of extracts from normal (control) and EIF4EBP1 knockout (KO) HeLa cells, using EIF4EBP1 antibody (144-01248) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - [KO Validated] EIF4EBP1 Polyclonal Antibody (144-01248)

Immunofluorescence analysis of NIH/3T3 cells using EIF4EBP1 antibody (144-01248) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] EIF4EBP1 Polyclonal Antibody (144-01248)

Expiration:

12 months from the date of shipment when stored properly.

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