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Anti-EIF4G1 Antibody
Catalog #: 144-07552
EIF4G1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q04637 |
| Gene Id | 1981 |
| Gene Symbols | EIF4G1 |
| Protein Name / Synonyms | EIF4G1, EIF-4G1, EIF4F, EIF4G, EIF4GI, P220, PARK18, eukaryotic translation initiation factor 4 gamma 1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
154kDa/158kDa/166kDa/171kDa/175kDa/176kDa
Observed MW
230kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 400-500 of human EIF4G1 (NP_886553.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of THP-1 cells, using EIF4G1 Antibody (144-07552) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver injury using EIF4G1 Antibody (144-07552) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using EIF4G1 Antibody (144-07552) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using EIF4G1 Antibody (144-07552) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using EIF4G1 antibody (144-07552). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.