Anti-EIF5A Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P63241
Gene Id	1984
Gene Symbols	EIF5A
Protein Name / Synonyms	EIF5A, EIF-5A, EIF5A1, eIF5AI, eukaryotic translation initiation factor 5A
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

16kDa/20kDa

Observed MW

17kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-154 of human EIF5A (NP_001137234.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using EIF5A antibody (144-02016) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - EIF5A Polyclonal Antibody (144-02016)

Western blot analysis of extracts of various cells, using EIF5A antibody (144-02016) at 1:1000 dilution ratio through one-step method.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
Western blot - EIF5A Polyclonal Antibody (144-02016)

Immunohistochemistry of paraffin-embedded human kidney using EIF5A antibody (144-02016) at dilution of 1:100 (40x lens).
Immunohistochemistry - EIF5A Polyclonal Antibody (144-02016)

Expiration:

12 months from the date of shipment when stored properly.

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