Anti-ELAC2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9BQ52
Gene Id	60528
Gene Symbols	ELAC2
Protein Name / Synonyms	ELAC2, COXPD17, ELC2, HPC2, elaC ribonuclease Z 2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Research Area	Cardiovascular Disease, Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene has a C-terminal domain with tRNA 3' processing endoribonuclease activity, which catalyzes the removal of the 3' trailer from precursor tRNAs. The protein also interacts with activated Smad family member 2 (Smad2) and its nuclear partner forkhead box H1 (also known as FAST-1), and reduced expression can suppress transforming growth factor-beta induced growth arrest. Mutations in this gene result in an increased risk of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100

Format

Calculated MW

50kDa/56kDa/87kDa/92kDa

Observed MW

105kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ELAC2 (NP_776065.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ELAC2 Antibody (144-07128) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - ELAC2 Polyclonal Antibody (144-07128)

Immunohistochemistry of paraffin-embedded human esophagus using ELAC2 antibody (144-07128) at dilution of 1:100 (40x lens).
Immunohistochemistry - ELAC2 Polyclonal Antibody (144-07128)

Immunofluorescence analysis of MCF7 cells using ELAC2 antibody (144-07128). Blue: DAPI for nuclear staining.
Immunofluorescence - ELAC2 Polyclonal Antibody (144-07128)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products