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Anti-ELAVL3 Antibody
Catalog #: 144-06091
ELAVL3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q14576 |
| Gene Id | 1995 |
| Gene Symbols | ELAVL3 |
| Protein Name / Synonyms | ELAVL3, HUC, HUCL, PLE21, ELAV-like protein 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
A member of the ELAVL protein family, ELAV-like 3 is a neural-specific RNA-binding protein which contains three RNP-type RNA recognition motifs. The observation that ELAVL3 is one of several Hu antigens (neuronal-specific RNA-binding proteins) recognized by the anti-Hu serum antibody present in sera from patients with paraneoplastic encephalomyelitis and sensory neuronopathy (PEM/PSN) suggests it has a role in neurogenesis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
38kDa/39kDa
Observed MW
37kDa
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ELAVL3 (NP_001411.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ELAVL3 antibody (144-06091) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat brain using ELAVL3 antibody (144-06091) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using ELAVL3 antibody (144-06091) at dilution of 1:200 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.