RayBiotech

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ISO 13485:2016 cGMP

Anti-ELMO1 Antibody

Catalog #: 144-60813

ELMO1 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
Q92556
Gene Id
9844
Gene Symbols
ELMO1
Protein Name / Synonyms
ELMO1, CED-12, CED12, ELMO-1, engulfment and cell motility 1
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes a member of the engulfment and cell motility protein family. These proteins interact with dedicator of cytokinesis proteins to promote phagocytosis and cell migration. Increased expression of this gene and dedicator of cytokinesis 1 may promote glioma cell invasion, and single nucleotide polymorphisms in this gene may be associated with diabetic nephropathy. Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
28kDa/49kDa/83kDa
Observed MW
72kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 527-727 of human ELMO1 (NP_055615.8).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ELMO1 antibody (144-014830) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
Western blot - ELMO1 antibody (144-014830)

Expiration:
12 months from the date of shipment when stored properly.