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Anti-ELMO3 Antibody
Catalog #: 144-07182
ELMO3 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q96BJ8 |
| Gene Id | 79767 |
| Gene Symbols | ELMO3 |
| Protein Name / Synonyms | ELMO3, CED-12, CED12, ELMO-3, engulfment and cell motility 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is similar to a C. elegans protein that functions in phagocytosis of apoptotic cells and in cell migration. Other members of this small family of engulfment and cell motility (ELMO) proteins have been shown to interact with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
68kDa/81kDa/87kDa
Observed MW
81kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 504-773 of human ELMO3 (NP_078988.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ELMO3 antibody (144-07182) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human kidney using ELMO3 antibody (144-07182) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.