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Anti-ENDOG Antibody
Catalog #: 144-00604
ENDOG Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q14249 |
| Gene Id | 2021 |
| Gene Symbols | ENDOG |
| Protein Name / Synonyms | ENDOG, endonuclease G |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Cancer, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a nuclear encoded endonuclease that is localized in the mitochondrion. The encoded protein is widely distributed among animals and cleaves DNA at GC tracts. This protein is capable of generating the RNA primers required by DNA polymerase gamma to initiate replication of mitochondrial DNA.
Dilutions
WB 1:200 - 1:500
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
32kDa
Observed MW
33kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 49-297 of human ENDOG (NP_004426.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ENDOG antibody (144-00604) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse kidney using ENDOG antibody (144-00604) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using ENDOG antibody (144-00604) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.