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Anti-ENO2 Antibody
Catalog #: 144-03118
ENO2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P09104 |
| Gene Id | 2026 |
| Gene Symbols | ENO2 |
| Protein Name / Synonyms | ENO2, HEL-S-279, NSE, enolase 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
42kDa/47kDa
Observed MW
50kDa
Immunogen
A synthetic peptide of human ENO2
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ENO2 antibody (144-03118) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat brain using ENO2 antibody (144-03118) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.