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Anti-ENO2 Antibody
Catalog #: 144-12341
ENO2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P09104 |
| Gene Id | 2026 |
| Gene Symbols | ENO2 |
| Protein Name / Synonyms | ENO2, HEL-S-279, NSE, enolase 2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
42kDa/47kDa
Observed MW
47kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 195-434 of human ENO2 (NP_001966.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ENO2 antibody (144-012341) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunofluorescence analysis of NIH/3T3 cells using ENO2 antibody (144-012341) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using ENO2 antibody (144-012341) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.