Anti-ENO3 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P13929
Gene Id	2027
Gene Symbols	ENO3
Protein Name / Synonyms	ENO3, GSD13, MSE, enolase 3
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.

Dilutions

WB 1:1000 - 1:4000
IHC 1:100 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

42kDa/44kDa/46kDa

Observed MW

47kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ENO3 (NP_001967.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ENO3 antibody (144-03852) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - ENO3 Polyclonal Antibody (144-03852)

Immunohistochemistry of paraffin-embedded rat testis using ENO3 antibody (144-03852) at dilution of 1:100 (40x lens).
Immunohistochemistry - ENO3 Polyclonal Antibody (144-03852)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products