Anti-EPCAM Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P16422
Gene Id	4072
Gene Symbols	EPCAM
Protein Name / Synonyms	EPCAM, DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, M4S1, MIC18, MK-1, TACSTD1, TROP1, epithelial cell adhesion molecule
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW

34kDa

Observed MW

40kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human EPCAM (NP_002345.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using EPCAM antibody (144-01177) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - EPCAM Polyclonal Antibody (144-01177)

Immunohistochemistry of paraffin-embedded human lung cancer using EPCAM antibody (144-01177) at dilution of 1:100 (40x lens).
Immunohistochemistry - EPCAM Polyclonal Antibody (144-01177)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products