Rabbit anti-Human/rat EpCAM Antibody

Does not react with mouse or rat. Other species not tested.

EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1

Epithelial cell adhesion molecule (Ep-CAM) (Adenocarcinoma-associated antigen) (Cell surface glycoprotein Trop-1) (Epithelial cell surface antigen) (Epithelial glycoprotein) (EGP) (Epithelial glycoprotein 314) (EGP314) (hEGP314) (KS 1/4 antigen) (KSA) (Major gastrointestinal tumor-associated protein GA733-2) (Tumor-associated calcium signal transducer 1) (CD antigen CD326)

A human recombinant protein was used as the immunogen for the EpCAM antibody.

Protein G affinity chromatography

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Western Blotting: 0.5-1ug/ml
IF: 1-2 ug/ml
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Flow Cytometry: 0.5-1ug/million cells in 0.1ml

Description

EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Storage

Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Expiration:

12 months from the date of shipment when stored properly.

Rabbit anti-Human/rat EpCAM Antibody

Does not react with mouse or rat. Other species not tested.

EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1

A human recombinant protein was used as the immunogen for the EpCAM antibody.

Protein G affinity chromatography

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Western Blotting: 0.5-1ug/ml
IF: 1-2 ug/ml
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Flow Cytometry: 0.5-1ug/million cells in 0.1ml

Description

Storage

Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Expiration:

12 months from the date of shipment when stored properly.

Size	20 µg, 100 µg
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Specificity	Does not react with mouse or rat. Other species not tested.
Host Species	Rabbit
Accession Number	P16422
Gene Id	4072
Gene Symbols	EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1
Protein Name / Synonyms	Epithelial cell adhesion molecule (Ep-CAM) (Adenocarcinoma-associated antigen) (Cell surface glycoprotein Trop-1) (Epithelial cell surface antigen) (Epithelial glycoprotein) (EGP) (Epithelial glycoprotein 314) (EGP314) (hEGP314) (KS 1/4 antigen) (KSA) (Major gastrointestinal tumor-associated protein GA733-2) (Tumor-associated calcium signal transducer 1) (CD antigen CD326)
Immunogen	A human recombinant protein was used as the immunogen for the EpCAM antibody.
Isotype	IgG
Purity	Protein G affinity chromatography
Concentration (lot specific)	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Recommended Applications	Western Blotting: 0.5-1ug/ml IF: 1-2 ug/ml Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT Flow Cytometry: 0.5-1ug/million cells in 0.1ml
Domain / Region / Terminus	Cell surface, cytoplasmic
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	≤-20°C

Size	20 µg, 100 µg
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Specificity	Does not react with mouse or rat. Other species not tested.
Host Species	Rabbit
Accession Number	P16422
Gene Id	4072
Gene Symbols	EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1
Protein Name / Synonyms	Epithelial cell adhesion molecule (Ep-CAM) (Adenocarcinoma-associated antigen) (Cell surface glycoprotein Trop-1) (Epithelial cell surface antigen) (Epithelial glycoprotein) (EGP) (Epithelial glycoprotein 314) (EGP314) (hEGP314) (KS 1/4 antigen) (KSA) (Major gastrointestinal tumor-associated protein GA733-2) (Tumor-associated calcium signal transducer 1) (CD antigen CD326)
Immunogen	A human recombinant protein was used as the immunogen for the EpCAM antibody.
Isotype	IgG
Purity	Protein G affinity chromatography
Concentration (lot specific)	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Recommended Applications	Western Blotting: 0.5-1ug/ml IF: 1-2 ug/ml Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT Flow Cytometry: 0.5-1ug/million cells in 0.1ml
Domain / Region / Terminus	Cell surface, cytoplasmic
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	≤-20°C

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