RayBiotech

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Anti-EPHA3 Antibody

Catalog #: 144-08414

EPHA3 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P29320
Gene Id
2042
Gene Symbols
EPHA3
Protein Name / Synonyms
EPHA3, EK4, ETK, ETK1, HEK, HEK4, TYRO4, EPH receptor A3
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IHC
Research AreaCancer
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Two alternatively spliced transcript variants have been described for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW
60kDa/110kDa
Observed MW
110kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 400-545 of human EPHA3 (NP_005224.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using EPHA3 antibody (144-08414) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - EPHA3 antibody (144-08414)

Immunohistochemistry of paraffin-embedded rat brain using EPHA3 antibody (144-08414) at dilution of 1:100 (40x lens).
Immunohistochemistry - EPHA3 antibody (144-08414)

Expiration:
12 months from the date of shipment when stored properly.