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Anti-EPO Antibody
Catalog #: 144-05663
EPO Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P01588 |
| Gene Id | 2056 |
| Gene Symbols | EPO |
| Protein Name / Synonyms | EPO, EP, MVCD2, erythropoietin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
Dilutions
WB 1:500 - 1:2000
IHC 1:100 - 1:200
IHC 1:100 - 1:200
Format
Calculated MW
21kDa
Observed MW
30kDa
Immunogen
A synthetic peptide of human EPO
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EPO antibody (144-05663) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human esophagus using EPO antibody (144-05663) (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using EPO antibody (144-05663) (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using EPO antibody (144-05663) (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using EPO antibody (144-05663) (40x lens).
Expiration:
12 months from the date of shipment when stored properly.