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Anti-EPPIN Antibody
Catalog #: 144-02149
EPPIN Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O95925 |
| Gene Id | 57119 |
| Gene Symbols | EPPIN |
| Protein Name / Synonyms | EPPIN, CT71, CT72, SPINLW1, WAP7, WFDC7, dJ461P17.2, eppin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes an epididymal protease inhibitor, which contains both kunitz-type and WAP-type four-disulfide core (WFDC) protease inhibitor consensus sequences. Most WFDC genes are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene is a member of the WFDC gene family and belongs to the telomeric cluster. The protein can inhibit human sperm motility and exhibits antimicrobial activity against E. coli, and polymorphisms in this gene are associated with male infertility. Read-through transcription also exists between this gene and the downstream WFDC6 (WAP four-disulfide core domain 6) gene. Two transcript variants encoding different isoforms have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IF 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
13kDa/15kDa/20kDa
Observed MW
25kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 22-133 of human EPPIN (NP_065131.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of mouse testis, using EPPIN antibody (144-02149) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Immunofluorescence analysis of HepG2 cells using EPPIN antibody (144-02149). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.