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Anti-ERBB2 Antibody
Catalog #: 102-17485
Rabbit Anti-Human ERBB2 Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P04626 |
| Gene Id | 2064 |
| Gene Symbols | ERBB2 |
| Protein Name / Synonyms | ERBB2 |
| Clonality | Polyclonal |
| Preparation | This ErbB2 antibody is generated from rabbits immunized with human recombinant ErbB2 protein. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC, IF |
| Research Area | Angiogenesis, Cardiovascular Disease, Cancer, Tyrosine Kinase Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
ErbB2, a member of the EGF receptor family, is an essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. This protein is not activated by EGF, TGF-alpha and amphiregulin. ErbB2 potentially forms a heterodimer with each of the other ERBB receptors. An interaction with PRKCABP has been suggested. Ligand-binding to this Type I membrane protein may increase phosphorylation on tyrosine residues.
Specificity
Other Names
ERBB2; HER2; MLN19; NEU; NGL; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2; p185erbB2; CD_antigen=CD340; Flags: Precursor
NCBI Accession #
NP_001005862.1;NP_004439.2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
137910
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50; IF: 1:10-50
 | Western blot analysis of HER2(arrow) using rabbit polyclonal HER2 antibody(Cat# 102-17485). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HER2 gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with HER2 antibody (Cat# 102-17485), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of MCF-7 cells using HER2 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Confocal immunofluorescent analysis of ErbB2 antibody (Cat# 102-17485) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.