Anti-ERBB4 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Rat
Host Species	Rabbit
Accession Number	Q15303
Gene Id	2066
Gene Symbols	ERBB4
Protein Name / Synonyms	ERBB4, ALS19, HER4, p180erbB4, erb-b2 receptor tyrosine kinase 4
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Research Area	Cardiovascular Disease, Cancer, Tyrosine Kinase Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

143kDa/145kDa/146kDa

Observed MW

150kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1150-1250 of human ERBB4 (NP_005226.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of rat brain, using ERBB4 antibody (144-00749) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - ERBB4 Polyclonal Antibody (144-00749)

Expiration:

12 months from the date of shipment when stored properly.

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