Anti-ERCC4 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q92889
Gene Id	2072
Gene Symbols	ERCC4
Protein Name / Synonyms	ERCC4, ERCC11, FANCQ, RAD1, XFEPS, XPF, DNA repair endonuclease XPF
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IP
Research Area	Cancer, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

The protein encoded by this gene forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in this gene are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6).

Dilutions

WB 1:500 - 1:2000
IP 1:20 - 1:50

Format

Calculated MW

42kDa/104kDa

Observed MW

120kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600-700 of human ERCC4 (NP_005227.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ERCC4 antibody (144-08119) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - ERCC4 antibody (144-08119)

Immunoprecipitation analysis of 150ug extracts of 293T cells using 3ug ERCC4 antibody (144-08119). Western blot was performed from the immunoprecipitate using ERCC4 antibody (144-08119) at a dilition of 1:1000.
Immunoprecipitation - 3ug ERCC4 antibody (144-08119)

Expiration:

12 months from the date of shipment when stored properly.

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