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Anti-ERCC8 (internal) Antibody
Catalog #: 107-10186
ERCC8 antibody [N2C2], Internal, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 1161 |
| Protein Name / Synonyms | ERCC8, Internal |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, Functional Assay, Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
CKN1 antibody, CSA antibody, ERCC8 antibody, Cockayne syndrome WD-repeat protein CSA antibody, DNA excision repair protein ERCC-8 antibody, cockayne syndrome WD repeat protein CSA antibody, excision repair cross-complementing rodent repair deficiency, complementation group 8 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
44 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 106 and 330 of CSA (Uniprot ID# Q13216)
Applications
Applications Tested
ICC/IF,Functional Assay,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
ERCC8 antibody [N2C2], Internal detects ERCC8 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg Raw264.7 whole cell lysate/extract
F. 30 µg C2C12 whole cell lysate/extract
10 % SDS-PAGE
ERCC8 antibody [N2C2], Internal (107-10186) dilution: 1:1000
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg Raw264.7 whole cell lysate/extract
F. 30 µg C2C12 whole cell lysate/extract
10 % SDS-PAGE
ERCC8 antibody [N2C2], Internal (107-10186) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ERCC8(CSA)(107-10186) antibody at 1:200 dilution.
Sample(30 µg of whole cell lysate)
A:293T
B:A431
12% SDS PAGE
107-10186 diluted at 1:500
A:293T
B:A431
12% SDS PAGE
107-10186 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.