[KO Validated] ERP29 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P30040 |
| Gene Id | 10961 |
| Gene Symbols | ERP29 |
| Protein Name / Synonyms | ERP29, C12orf8, ERp28, ERp31, HEL-S-107, PDI-DB, PDIA9, endoplasmic reticulum protein 29 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a protein which localizes to the lumen of the endoplasmic reticulum (ER). It is a member of the protein disulfide isomerase (PDI) protein family but lacks an active thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase. The canonical protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
5kDa/28kDa
Observed MW
29kDa
Immunogen
A synthetic peptide of human ERP29
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ERP29 antibody (144-07959) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.![Western blot - [KO Validated] ERP29 Polyclonal Antibody (144-07959)](https://cdn.sanity.io/images/g51potcz/production/9d9f3512b6edb96a25c1df8cf31c0a40d9ade32c-608x350.jpg)
Western blot analysis of extracts from normal (control) and ERP29 knockout (KO) 293T cells, using ERP29 antibody (144-07959) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.![Western blot - [KO Validated] ERP29 Polyclonal Antibody (144-07959)](https://www.raybiotech.com/files/images/Antibodies/144/144-07959/2.jpg)
Expiration:
12 months from the date of shipment when stored properly.