Anti-ESD Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P10768
Gene Id	2098
Gene Symbols	ESD
Protein Name / Synonyms	ESD, FGH, esterase D
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a serine hydrolase that belongs to the esterase D family. The encoded enzyme is active toward numerous substrates including O-acetylated sialic acids, and it may be involved in the recycling of sialic acids. This gene is used as a genetic marker for retinoblastoma and Wilson's disease.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

31kDa

Observed MW

36kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-282 of human ESD (NP_001975.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ESD antibody (144-06228) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - ESD Polyclonal Antibody (144-06228)

Immunohistochemistry of paraffin-embedded Human trachea using ESD antibody (144-06228) at dilution of 1:100 (40x lens).
Immunohistochemistry - ESD Polyclonal Antibody (144-06228)

Immunohistochemistry of paraffin-embedded mouse lung using ESD antibody (144-06228) at dilution of 1:100 (40x lens).
Immunohistochemistry - ESD Polyclonal Antibody (144-06228)

Expiration:

12 months from the date of shipment when stored properly.

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