Anti-ESR1/ER (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P03372
Gene Id	2099
Gene Symbols	ESR1
Protein Name / Synonyms	ESR1/ER (Center)
Clonality	Polyclonal
Preparation	This ESR1/ER antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-273 amino acids from the Central region of human ESR1/ER.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Pig

Other Names

ESR1; ESR; NR3A1; Estrogen receptor; ER-alpha; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1

NCBI Accession #

NP_000116.2;NP_001116212.1;NP_001116213.1;NP_001116214.1

Other Accession #

P06211; Q29040; P19785; P49884; NP_001116213.1; NP_000116.2; NP_001116214.1; NP_001116212.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

66216

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50

	ESR1/ER Antibody (Center) (Cat# 102-12052) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the ESR1/ER antibody detected the ESR1/ER protein (arrow).
	ESR1/ER Antibody (Center) (Cat# 102-12052)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ESR1/ER Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	ESR1/ER Antibody (Center) (Cat# 102-12052)immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ESR1/ER Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of ESR1/ER Antibody (Center)(Cat# 102-12052) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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