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Anti-Estrogen Receptor Antibody
Catalog #: 188-10079
Mouse anti-Human Estrogen Receptor Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | Other species not known |
| Host Species | Mouse |
| Accession Number | P03372 |
| Gene Id | 2099 |
| Gene Symbols | ESR1,ESR,NR3A1 |
| Protein Name / Synonyms | Estrogen receptor (ER) (ER-alpha) (Estradiol receptor) (Nuclear receptor subfamily 3 group A member 1) |
| Immunogen | Recombinant human Estrogen Receptor alpha protein (aa 2-185) was used as the immunogen for this antibody. |
| Isotype | Kappa, IgG1 |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 0.2 mg/ml in 1X PBS (pH 7.4) with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
| Recommended Applications | Western Blotting: use antibody at 0.5-1ug/ml IHC (FFPE): 1-2ug/ml for 30 minutes at RT (1) Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Nuclear |
| Research Area | Inflammation, Endocrine |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Estrogen receptor (ER) is an important regulator of growth and differentiation in the mammary gland. It is found in two forms, alpha and beta. Both forms are widely expressed, however, alpha is the predominant form in endometrium and breast cancer cells, and beta is more highly expressed in brain, kidney, heart, lung and bone. Activated Estrogen receptor dimerizes and forms both homo- and heterodimers. ER contains five domains: A-F. The A and B domain are able to weakly activate ER in the absence of ligand. The C domain binds DNA and the E domain contains the ligand binding site. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. This antibody is specific to estrogen receptor alpha and shows minimal cross-reaction with other members of the family.
Storage
Store the Estrogen Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.