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Anti-Estrogen Receptor beta Antibody, Sodium Azide Free
Catalog #: 188-10351-SAF
Mouse anti-Human Estrogen Receptor beta Antibody [Sodium Azide Free]
Product Description
Specifications
| Size | 100 µg |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Specificity | Other species not tested. |
| Host Species | Mouse |
| Accession Number | Q92731 |
| Gene Id | 2100 |
| Gene Symbols | ESR2,ESTRB,NR3A2 |
| Protein Name / Synonyms | Estrogen receptor beta (ER-beta) (Nuclear receptor subfamily 3 group A member 2) |
| Immunogen | A C-terminus fragment of recombinant human ER-b protein was used as the immunogen for the Estrogen Receptor beta antibody. |
| Isotype | IgG2a |
| Purity | Protein G affinity chromatography |
| Concentration (lot specific) | 1 mg/ml in 1X PBS, BSA free, sodium azide free |
| Recommended Applications | Flow Cytometry: 0.5-1ug/million cells in 0.1ml Immunofluorescence: 0.5-1ug/ml Immunohistochemistry (Formalin-fixed):0.5-1ug/ml for 30 min at RT (1) Western blot: 0.5-1ug/ml Prediluted format: incubate for 30 min at RT (2) |
| Domain / Region / Terminus | Predominantly nuclear |
| Research Area | Endocrine |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Description
Description
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics.
Storage
Store the Estrogen Receptor beta antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Expiration:
12 months from the date of shipment when stored properly.