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Anti-ETS1 (N-term) Antibody
Catalog #: 102-10771
Rabbit Anti-Human ETS1 (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P14921 |
| Gene Id | 2113 |
| Gene Symbols | ETS1 |
| Protein Name / Synonyms | ETS1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This ETS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-70 amino acids from the N-terminal region of human ETS1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF, FC |
| Research Area | Angiogenesis, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
ETS transcriptions factors, such as ETS1, regulate numerous genes and are involved in stem cell development, cell senescence and death, and tumorigenesis. The conserved ETS domain within these proteins is a winged helix-turn-helix DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T of target genes (summary by Dwyer et al., 2007 [PubMed 17986575]).
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
ETS1; EWSR2; Protein C-ets-1; p54
NCBI Accession #
NP_001137292.1;NP_001155894.1;NP_005229.1
Other Accession #
P41156; P27577; NP_005229.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
50408
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IF: 1:10-50
 | Western blot analysis of lysate from Jurkat cell line, using ETS1 Antibody (N-term) (Cat# 102-10771). 102-10771 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug. |
 | ETS1 Antibody (N-term) (Cat# 102-10771) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Confocal immunofluorescent analysis of ETS1 Antibody (N-term)(Cat# 102-10771) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.