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Anti-ETV4 (C-term) Antibody

Catalog #: 102-16863

Rabbit Anti-ETV4 (C-term) Antibody, 400 ul

Product Description

Specifications

Size400 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
P43268
Gene Id
2118
Gene Symbols
ETV4
Protein Name / Synonyms
ETV4 (C-term)
ClonalityPolyclonal
PreparationThis ETV4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 419-447 amino acids from the C-terminal region of human ETV4.
Concentration (lot specific)
2.0 mg/ml
Recommended ApplicationsWestern Blotting, IHC-P, FC
Shipping TypeBlue ice
Storage-20°C

Description

Introduction

ETV4 is a transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.

Specificity

Other Names
ETV4; E1AF; PEA3; ETS translocation variant 4; Adenovirus E1A enhancer-binding protein; E1A-F; Polyomavirus enhancer activator 3 homolog
NCBI Accession #
NP_001073143.1;NP_001248366.1;NP_001248367.1;NP_001977.1
Other Accession #
P28322

Format

Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
53938
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
Western blot analysis of ETV4 antibody (C-term) (Cat# 102-16863) in mouse heart tissue lysates (35ug/lane). ETV4 (arrow) was detected using the purified Pab.
Western blot analysis of ETV4 (arrow) using rabbit polyclonal ETV4 Antibody (C-term) (RB20008). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ETV4 gene (Lane 2) .
Formalin-fixed and paraffin-embedded human brain tissue reacted with ETV4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of widr cells using ETV4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.