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Anti-EXT1 Antibody
Catalog #: 144-02030
EXT1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16394 |
| Gene Id | 2131 |
| Gene Symbols | EXT1 |
| Protein Name / Synonyms | EXT1, EXT, LGCR, LGS, TRPS2, TTV, exostosin-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cardiovascular Disease, Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes an endoplasmic reticulum-resident type II transmembrane glycosyltransferase involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type I form of multiple exostoses.
Dilutions
WB 1:500 - 1:2000
IF 1:10 - 1:100
IF 1:10 - 1:100
Format
Calculated MW
86kDa
Observed MW
88kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 467-746 of human EXT1 (NP_000118.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EXT1 antibody (144-02030) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using EXT1 antibody (144-02030). Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.