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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O43909 |
| Gene Id | 2137 |
| Gene Symbols | EXTL3 |
| Protein Name / Synonyms | EXTL3, BOTV, EXTL1L, EXTR1, REGR, RPR, ISDNA, exostosin-like 3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a single-pass membrane protein which functions as a glycosyltransferase. The encoded protein catalyzes the transfer of N-acetylglucosamine to glycosaminoglycan chains. This reaction is important in heparin and heparan sulfate synthesis. Alternative splicing results in the multiple transcript variants.
Dilutions
WB 1:200 - 1:2000
Format
Calculated MW
104kDa
Observed MW
105kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 52-220 of human EXTL3 (NP_001431.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EXTL3 antibody (144-03857) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Expiration:
12 months from the date of shipment when stored properly.