3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-EZH2 Antibody
Catalog #: 144-11085
EZH2 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | Q15910 |
| Gene Id | 2146 |
| Gene Symbols | EZH2 |
| Protein Name / Synonyms | EZH2, ENX-1, ENX1, EZH1, EZH2b, KMT6, KMT6A, WVS, WVS2, histone-lysine N-methyltransferase EZH2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
79kDa/81kDa/84kDa/85kDa/86kDa
Observed MW
85kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human EZH2 (NP_001190176.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using EZH2 antibody (144-011085) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat testis using EZH2 antibody (144-011085) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using EZH2 antibody (144-011085) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using EZH2 antibody (144-011085) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EZH2 antibody (144-011085) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using EZH2 antibody (144-011085) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using EZH2 antibody (144-011085) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using EZH2 antibody (144-011085).
Expiration:
12 months from the date of shipment when stored properly.