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Anti-FABP2 Antibody

Catalog #: 144-01621

FABP2 Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P12104
Gene Id
2169
Gene Symbols
FABP2
Protein Name / Synonyms
FABP2, FABPI, I-FABP, fatty acid binding protein 2
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Shipping TypeBlue ice
Storage-20°C

Description

Background

The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. This gene has a polymorphism at codon 54 that identified an alanine-encoding allele and a threonine-encoding allele. Thr-54 protein is associated with increased fat oxidation and insulin resistance.

Dilutions

WB 1:500 - 1:2000
IF 1:10 - 1:100

Format

Calculated MW
15kDa
Observed MW
15kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-132 of human FABP2 (NP_000125.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using FABP2 antibody (144-01621) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - FABP2 Polyclonal Antibody (144-01621)

Immunofluorescence analysis of HeLa cells using FABP2 antibody (144-01621). Blue: DAPI for nuclear staining.
Immunofluorescence - FABP2 Polyclonal Antibody (144-01621)

Expiration:
12 months from the date of shipment when stored properly.