Factor VII antibody [N3C3], 0.74 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 2155 |
| Protein Name / Synonyms | Factor VII |
| Concentration (lot specific) | 0.74 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
F7 antibody, SPCA antibody, proconvertin antibody, FVII coagulation protein antibody, coagulation factor VII antibody, eptacog alfa antibody, serum prothrombin conversion accelerator antibody, coagulation factor VII (serum prothrombin conversion accelerator) antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
52 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 209 and 466 of Factor VII (Uniprot ID# P08709)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded normal gastric epithelium (gland) , using F7(107-10250) antibody at 1:100 dilution.
Sample(30 µg of whole cell lysate)
A:H1299
7.5% SDS PAGE
107-10250 diluted at 1:500
A:H1299
7.5% SDS PAGE
107-10250 diluted at 1:500
Sample (30 ug of whole cell lysate)
A:NIH-3T3
10% SDS PAGE
107-10250 diluted at 1:1000
A:NIH-3T3
10% SDS PAGE
107-10250 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.