FADD Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13158 |
| Gene Id | 8772 |
| Gene Symbols | FADD |
| Protein Name / Synonyms | FADD, GIG3, MORT1, Fas associated via death domain |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IP |
| Research Area | Apoptosis, Cardiovascular Disease, Cancer, Autoimmune Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:200
IHC 1:50 - 1:200
IP 1:50 - 1:200
Format
Calculated MW
23kDa
Observed MW
26kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-208 of human FADD (NP_003815.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using FADD antibody (144-05819) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded rat kidney using FADD antibody (144-05819) at dilution of 1:200 (40x lens).
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug FADD antibody (144-05819). Western blot was performed from the immunoprecipitate using FADD antibody (144-05819) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.