RayBiotech

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ISO 13485:2016 cGMP

Anti-FAH Antibody

Catalog #: 144-06586

FAH Polyclonal Antibody

Product Description

Specifications

Size20 µL, 100 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse, Rat
Host SpeciesRabbit
Accession Number
P16930
Gene Id
2184
Gene Symbols
FAH
Protein Name / Synonyms
FAH, fumarylacetoacetase
ClonalityPolyclonal
IsotypeIgG
Purity
Affinity purification
BuffersPBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended ApplicationsWestern Blotting, IF
Research AreaCancer
Shipping TypeBlue ice
Storage-20°C

Description

Background

This gene encodes the last enzyme in the tyrosine catabolism pathway. FAH deficiency is associated with Type 1 hereditary tyrosinemia (HT).

Dilutions

WB 1:500 - 1:2000
IF 1:10 - 1:100

Format

Calculated MW
38kDa/46kDa
Observed MW
45kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-419 of human FAH (NP_000128.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using FAH antibody (144-06586) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Western blot - FAH antibody (144-06586)

Immunofluorescence analysis of A549 cells using FAH antibody (144-06586). Blue: DAPI for nuclear staining.
Immunofluorescence - FAH antibody (144-06586)

Expiration:
12 months from the date of shipment when stored properly.