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Anti-FANCA Antibody
Catalog #: 144-07671
FANCA Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O15360 |
| Gene Id | 2175 |
| Gene Symbols | FANCA |
| Protein Name / Synonyms | FANCA, FA, FA-H, FA1, FAA, FACA, FAH, FANCH, Fanconi anemia group A protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group A. Alternative splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are the most common cause of Fanconi anemia.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
32kDa/159kDa/162kDa
Observed MW
163kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-275 of human FANCA (NP_000126.2).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of HeLa cells, using FANCA antibody (144-07671) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using FANCA antibody (144-07671) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.