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Anti-FASN Antibody
Catalog #: 144-00461
FASN Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P49327 |
| Gene Id | 2194 |
| Gene Symbols | FASN |
| Protein Name / Synonyms | FASN, FAS, OA-519, SDR27X1, fatty acid synthase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Format
Calculated MW
273kDa
Observed MW
280kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human FASN (NP_004095.4).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using FASN antibody (144-00461) at 1:200 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver cancer using FASN Antibody (144-00461) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using FASN Antibody (144-00461) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using FASN antibody (144-00461). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug FASN antibody (144-00461). Western blot was performed from the immunoprecipitate using FASN antibody (144-00461) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.